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Cogenics Inc
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Image Search Results
Journal: PLoS ONE
Article Title: Identification of Heat Responsive Genes in Brassica napus Siliques at the Seed-Filling Stage through Transcriptional Profiling
doi: 10.1371/journal.pone.0101914
Figure Lengend Snippet: Relative expression levels of 18 up-regulated genes were detected in the SW and seeds sampled at 24 h (A and B) and 48 h (C and D) after heat treatment. 10 down-regulated genes, of which 5 involving glucosinolate metabolism and another 5 associated with flavonoid synthesis were analyzed in the SW (E) and seeds (F) at 24 h after treatment respectively. Two genes may responsible for lipid synthesis were also detected in seeds (G). (H) Correlation of the gene expression ratios obtained from qRT-PCR and microarray data. The qRT-PCR log 2 value of the expression ratio (y-axis) has been plotted against the value from the microarray (x-axis). The results of all the tested genes were listed in detail in . Data were collected from three biological replicates and three technical replicates for each sample.
Article Snippet: To identify genes responsive to heat stress in oilseed rape at the seed-filling stage, a
Techniques: Expressing, Gene Expression, Quantitative RT-PCR, Microarray